- Catalog number
- EK0413
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Sample 3 234 249 255 221 239 13.25 5.5%
- Synonyms
- Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8) 77; GCP/IL-8 protein II; IL-8 (1-77) ; IL8/NAP1 form II; MDNCF-b; IL-8 (5-77) ; IL-8 (6-77) ; (Ser-IL-8) 72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77) ; GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77) ; GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77) ; GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8
- Gene Name
- CXCL8
- Gene ID
- 3576
- UniProt
- P10145
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S28-S99
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6%
- Sensitivity
- <1 pg/mL
- Function
- IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11098 MW
- Specificity
- Natural and recombinant human IL-8
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-8
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- Interleukin-8
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
