- Catalog number
- EK0417
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 38 38 37 36 2.06 5.7% Sample 2 219 193 202 190 201 11.29 5.6% Sample 3 466 448 405 427 436 22.83 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 38 38 37 36 2.06 5.7% Sample 1 33 38 38 37 36 2.06 5.7% Sample 2 219 193 202 190 201 11.29 5.6% Sample 2 219 193 202 190 201 11.29 5.6% Sample 3 466 448 405 427 436 22.83 5.2% Sample 3 466 448 405 427 436 22.83 5.2%
- Synonyms
- Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10
- Gene Name
- IL10
- Gene ID
- 16153
- UniProt
- P18893
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S19-S178
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 219 466 35 203 420 Standard deviation 1.88 14.01 19.1 2.66 16.64 25.7 CV (%) 5.7% 6.4% 4.1% 7.6% 8.2% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 219 466 35 203 420 Standard deviation 1.88 14.01 19.1 2.66 16.64 25.7 CV (%) 5.7% 6.4% 4.1% 7.6% 8.2% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 219 466 35 203 420 Mean (pg/ml) 33 219 466 35 203 420 Standard deviation 1.88 14.01 19.1 2.66 16.64 25.7 Standard deviation 1.88 14.01 19.1 2.66 16.64 25.7 CV (%) 5.7% 6.4% 4.1% 7.6% 8.2% 6% CV (%) 5.7% 6.4% 4.1% 7.6% 8.2% 6%
- Sensitivity
- <1 pg/mL
- Function
- Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20641 MW
- Specificity
- Natural and recombinant mouse IL-10
- Gene Name Synonym
- Interleukin-10
- Subcellular Location
- Secreted.
- Protein Name
- Interleukin-10
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.101 0.143 0.245 0.417 0.735 1.265 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.101 0.143 0.245 0.417 0.735 1.265 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.101 0.143 0.245 0.417 0.735 1.265 2.059 O.D. 0.062 0.101 0.143 0.245 0.417 0.735 1.265 2.059
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
