Mouse IL-13/Interleukin-13 ELISA Kit PicoKine®

About this product

Mouse IL-13 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0425

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin (IL) -13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 38 36 37 2.23 6% Sample 2 209 191 179 193 193 10.67 5.5% Sample 3 526 501 535 503 516 14.61 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 38 36 37 2.23 6% Sample 1 40 34 38 36 37 2.23 6% Sample 2 209 191 179 193 193 10.67 5.5% Sample 2 209 191 179 193 193 10.67 5.5% Sample 3 526 501 535 503 516 14.61 2.8% Sample 3 526 501 535 503 516 14.61 2.8%

Synonyms

Interleukin-13; IL-13; T-cell activation protein P600; Il13; Il-13

Gene Name

IL13

Gene ID

16163

UniProt

P20109

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S26-F131

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 209 526 36 193 532 Standard deviation 2.68 15.46 30.5 2.41 16.79 32.45 CV (%) 6.7% 7.4% 5.8% 6.7% 8.7% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 209 526 36 193 532 Standard deviation 2.68 15.46 30.5 2.41 16.79 32.45 CV (%) 6.7% 7.4% 5.8% 6.7% 8.7% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 209 526 36 193 532 Mean (pg/ml) 40 209 526 36 193 532 Standard deviation 2.68 15.46 30.5 2.41 16.79 32.45 Standard deviation 2.68 15.46 30.5 2.41 16.79 32.45 CV (%) 6.7% 7.4% 5.8% 6.7% 8.7% 6.1% CV (%) 6.7% 7.4% 5.8% 6.7% 8.7% 6.1%

Sensitivity

<2 pg/mL

Function

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14108 MW

Specificity

Natural and recombinant mouse IL-13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-13

Subcellular Location

Secreted.

Protein Name

Interleukin-13

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.068 0.135 0.266 0.507 0.969 1.516 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.068 0.135 0.266 0.507 0.969 1.516 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.068 0.135 0.266 0.507 0.969 1.516 2.029 O.D. 0.008 0.068 0.135 0.266 0.507 0.969 1.516 2.029

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.