- Catalog number
- EK0428
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 64 55 56 58 3.5 6% Sample 2 213 197 219 232 215 12.57 5.8% Sample 3 983 978 906 908 943 36.79 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 64 55 56 58 3.5 6% Sample 1 59 64 55 56 58 3.5 6% Sample 2 213 197 219 232 215 12.57 5.8% Sample 2 213 197 219 232 215 12.57 5.8% Sample 3 983 978 906 908 943 36.79 3.9% Sample 3 983 978 906 908 943 36.79 3.9%
- Synonyms
- Pro-interleukin-16; Interleukin-16; IL-16; Lymphocyte chemoattractant factor; LCF; IL16
- Gene Name
- IL16
- Gene ID
- 3603
- UniProt
- Q14005
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: P1204-S1332
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7%
- Sensitivity
- <10 pg/mL
- Function
- Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 141752 MW
- Specificity
- Natural and recombinant human IL-16
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Pro-interleukin-16
- Subcellular Location
- Interleukin-16: Secreted.
- Sequence Similarities
- Contains 4 PDZ (DHR) domains.
- Protein Name
- Pro-interleukin-16
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
