- Catalog number
- EK0431
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13) . IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor, and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 37 38 37 1.22 3.2% Sample 2 181 172 153 155 165 11.71 7% Sample 3 523 460 470 520 493 28.49 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 37 38 37 1.22 3.2% Sample 1 38 35 37 38 37 1.22 3.2% Sample 2 181 172 153 155 165 11.71 7% Sample 2 181 172 153 155 165 11.71 7% Sample 3 523 460 470 520 493 28.49 5.7% Sample 3 523 460 470 520 493 28.49 5.7%
- Synonyms
- Interleukin 17A ; Il17a ; Il17 ; mCG_7914
- Gene Name
- IL17A
- Gene ID
- 16171
- UniProt
- Q544E6
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: T22-A158
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 181 523 37 176 485 Standard deviation 2.35 10.13 40.27 2.47 10.91 41.71 CV (%) 6.2% 5.6% 7.7% 6.7% 6.2% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 181 523 37 176 485 Standard deviation 2.35 10.13 40.27 2.47 10.91 41.71 CV (%) 6.2% 5.6% 7.7% 6.7% 6.2% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 181 523 37 176 485 Mean (pg/ml) 38 181 523 37 176 485 Standard deviation 2.35 10.13 40.27 2.47 10.91 41.71 Standard deviation 2.35 10.13 40.27 2.47 10.91 41.71 CV (%) 6.2% 5.6% 7.7% 6.7% 6.2% 8.6% CV (%) 6.2% 5.6% 7.7% 6.7% 6.2% 8.6%
- Sensitivity
- <1 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17490 MW
- Specificity
- Natural and recombinant mouse IL-17/IL-17A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin 17A
- Protein Name
- Interleukin-17A
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.116 0.168 0.275 0.483 0.818 1.503 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.116 0.168 0.275 0.483 0.818 1.503 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.116 0.168 0.275 0.483 0.818 1.503 2.232 O.D. 0.046 0.116 0.168 0.275 0.483 0.818 1.503 2.232
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
