- Catalog number
- EK0436
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 219 202 230 239 222 13.79 6.2% Sample 2 1790 1582 1652 1826 1712 99.47 5.8% Sample 3 4940 5199 5124 5484 5186 195.78 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 219 202 230 239 222 13.79 6.2% Sample 1 219 202 230 239 222 13.79 6.2% Sample 2 1790 1582 1652 1826 1712 99.47 5.8% Sample 2 1790 1582 1652 1826 1712 99.47 5.8% Sample 3 4940 5199 5124 5484 5186 195.78 3.7% Sample 3 4940 5199 5124 5484 5186 195.78 3.7%
- Synonyms
- Laminin subunit alpha-3; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; Lama3
- Gene Name
- LAMA1; LAMA2; LAMB1; LAMB2
- Gene ID
- 16772, 16773, 16774, 16775, 16776, 16777, 16779, 16780, 226519, 16782, 23928
- UniProt
- P19137, Q60675, Q61789, P97927, Q61001, P02469, Q61292, Q61087, P02468, Q61092, Q9R0B6
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Target protein isolated from murine sarcoma basement membrane
- Capture Antibody
- Polyclonal antibody from rabbit
- Detection Antibody
- Polyclonal antibody from rabbit
- Tissue Specificity
- Basal membrane of the upper alimentary tract and urinary and nasal epithelia, salivary glands and teeth (both variants) . Isoform A is predominantly expressed in skin, hair follicles and developing neurons of the trigeminal ganglion. Isoform B was found in bronchi, alveoli, stomach, intestinal crypts, whisker pads, CNS, telencephalic neuroectoderm, thalamus, Rathke pouch and periventricular subependymal germinal layer.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Neuroscience, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 219 1790 4940 238 1634 5253 Standard deviation 17.08 77.48 301.34 20.46 68.62 388.72 CV (%) 7.8% 4.2% 6.1% 8.6% 4.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 219 1790 4940 238 1634 5253 Standard deviation 17.08 77.48 301.34 20.46 68.62 388.72 CV (%) 7.8% 4.2% 6.1% 8.6% 4.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 219 1790 4940 238 1634 5253 Mean (pg/ml) 219 1790 4940 238 1634 5253 Standard deviation 17.08 77.48 301.34 20.46 68.62 388.72 Standard deviation 17.08 77.48 301.34 20.46 68.62 388.72 CV (%) 7.8% 4.2% 6.1% 8.6% 4.2% 7.4% CV (%) 7.8% 4.2% 6.1% 8.6% 4.2% 7.4%
- Sensitivity
- <10 pg/mL
- Function
- Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 366227 MW
- Specificity
- Natural mouse Laminin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Laminin subunit alpha-3
- Subcellular Location
- Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Sequence Similarities
- Contains 15 laminin EGF-like domains.
- Protein Name
- Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.074 0.131 0.236 0.444 0.798 1.388 2.055 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.074 0.131 0.236 0.444 0.798 1.388 2.055 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.074 0.131 0.236 0.444 0.798 1.388 2.055 O.D. 0.021 0.074 0.131 0.236 0.444 0.798 1.388 2.055
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
