- Catalog number
- EK0437
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 83 84 87 83 2.16 2.6% Sample 2 485 539 463 476 490 28.93 5.9% Sample 3 1057 1101 1114 1116 1097 23.8 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 83 84 87 83 2.16 2.6% Sample 1 81 83 84 87 83 2.16 2.6% Sample 2 485 539 463 476 490 28.93 5.9% Sample 2 485 539 463 476 490 28.93 5.9% Sample 3 1057 1101 1114 1116 1097 23.8 2.1% Sample 3 1057 1101 1114 1116 1097 23.8 2.1%
- Synonyms
- Leptin ; Obesity factor ; LEP ; hCG_33000 , tcag7.84
- Gene Name
- LEP
- Gene ID
- 3952
- UniProt
- A4D0Y8
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V22-C167
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Mesenchymal Stem Cells, Metabolism, Neurology Process, Neuroscience, Obesity, Signal Transduction, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7%
- Sensitivity
- <10 pg/mL
- Function
- May function as part of a signaling pathway that acts to regulate the size of the body fat depot. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 18641 MW
- Specificity
- Natural and recombinant human Leptin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Leptin
- Subcellular Location
- Secreted .
- Protein Name
- Leptin
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
