- Catalog number
- EK0442
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 34 30 33 2.27 6.8% Sample 2 152 155 149 171 156 8.49 5.4% Sample 3 451 459 443 431 446 10.34 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 34 30 33 2.27 6.8% Sample 1 35 36 34 30 33 2.27 6.8% Sample 2 152 155 149 171 156 8.49 5.4% Sample 2 152 155 149 171 156 8.49 5.4% Sample 3 451 459 443 431 446 10.34 2.3% Sample 3 451 459 443 431 446 10.34 2.3%
- Synonyms
- C-C motif chemokine 8; HC14; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; MCP-2 (6-76) ; CCL8; MCP2, SCYA10, SCYA8
- Gene Name
- CCL8
- Gene ID
- 6355
- UniProt
- P80075
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Q24-P99
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 152 451 36 154 486 Standard deviation 2.76 9.27 19.84 3.13 11.24 27.7 CV (%) 7.9% 6.1% 4.4% 8.7% 7.3% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 152 451 36 154 486 Standard deviation 2.76 9.27 19.84 3.13 11.24 27.7 CV (%) 7.9% 6.1% 4.4% 8.7% 7.3% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 152 451 36 154 486 Mean (pg/ml) 35 152 451 36 154 486 Standard deviation 2.76 9.27 19.84 3.13 11.24 27.7 Standard deviation 2.76 9.27 19.84 3.13 11.24 27.7 CV (%) 7.9% 6.1% 4.4% 8.7% 7.3% 5.7% CV (%) 7.9% 6.1% 4.4% 8.7% 7.3% 5.7%
- Sensitivity
- <1 pg/mL
- Function
- Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2 (6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11246 MW
- Specificity
- Natural and recombinant human CCL8
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 8
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Protein Name
- C-C motif chemokine 8
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.086 0.177 0.375 0.683 1.197 1.661 2.024 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.086 0.177 0.375 0.683 1.197 1.661 2.024 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.086 0.177 0.375 0.683 1.197 1.661 2.024 O.D. 0.011 0.086 0.177 0.375 0.683 1.197 1.661 2.024
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
