Rat MIP-3 Alpha/CCL20 ELISA Kit PicoKine®

About this product

Rat MIP-3 Alpha/CCL20 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Ccl20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0455

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20) . The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 88 98 96 93 3.76 4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 3 782 809 756 768 778 19.74 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 88 98 96 93 3.76 4% Sample 1 93 88 98 96 93 3.76 4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 3 782 809 756 768 778 19.74 2.5% Sample 3 782 809 756 768 778 19.74 2.5%

Synonyms

C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20; Ccl20; Scya20, St38

Gene Name

CCL20

Gene ID

29538

UniProt

P97884

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S27-M96

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Low levels in thymus and lung.

Applications

ELISA

Field of Research

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6%

Sensitivity

<1 pg/mL

Function

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (By similarity) . May play a role in modulating inflammatory cell recruitment to the CNS and therefore contribute to tissue injury in ischemic stroke and autoimmune diseases. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10875 MW

Specificity

Natural and recombinant rat MIP-3 alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 20

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 20

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.