- Catalog number
- EK0458
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% Sample 3 4510 5168 4653 4926 4814 253.07 5.2%
- Synonyms
- CDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7)
- Gene Name
- MMP1
- Gene ID
- 4312
- UniProt
- B4DN15
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: F20-N469
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6%
- Sensitivity
- <20 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 46266 MW
- Specificity
- Natural and recombinant human total MMP-1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Matrix metallopeptidase 1 (interstitial collagenase)
- Protein Name
- Interstitial collagenase
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
