- Catalog number
- EK0463
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively) . Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 250 279 270 12.09 4.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 3 5090 5484 5287 4635 5124 314.81 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 250 279 270 12.09 4.4% Sample 1 272 280 250 279 270 12.09 4.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 3 5090 5484 5287 4635 5124 314.81 6.1% Sample 3 5090 5484 5287 4635 5124 314.81 6.1%
- Synonyms
- Matrilysin;3.4.24.23; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase; MMP7; MPSL1, PUMP1
- Gene Name
- MMP7
- Gene ID
- 4316
- UniProt
- P09237
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L18-K267
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin), saliva and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2%
- Sensitivity
- <6 pg/mL
- Function
- Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 29677 MW
- Specificity
- Natural and recombinant human total MMP-7
- Anticoagulant
- Heparin
- Gene Name Synonym
- Matrilysin
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Protein Name
- Matrilysin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
