- Catalog number
- EK0465
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9) . The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 203 233 214 221 13.88 6.2% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 203 233 214 221 13.88 6.2% Sample 1 237 203 233 214 221 13.88 6.2% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4%
- Synonyms
- Matrix metalloproteinase-9; MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase; Gelatinase B; GELB;67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9; MMP9; CLG4B
- Gene Name
- MMP9
- Gene ID
- 4318
- UniProt
- P14780
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A20-D707
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Produced by normal alveolar macrophages and granulocytes.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin), saliva and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8%
- Sensitivity
- <5 pg/mL
- Function
- May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 78458 MW
- Specificity
- Natural and recombinant human total MMP-9
- Anticoagulant
- Heparin
- Gene Name Synonym
- Matrix metalloproteinase-9
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Protein Name
- Matrix metalloproteinase-9
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
