- Catalog number
- EK0466
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9) . The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 213 210 203 207 3.96 1.9% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 3 4289 3856 3877 4024 4011 172.79 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 213 210 203 207 3.96 1.9% Sample 1 205 213 210 203 207 3.96 1.9% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 3 4289 3856 3877 4024 4011 172.79 4.3% Sample 3 4289 3856 3877 4024 4011 172.79 4.3%
- Synonyms
- Matrix metalloproteinase-9; MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase; Gelatinase B; GELB; Mmp9; Clg4b
- Gene Name
- MMP9
- Gene ID
- 17395
- UniProt
- P41245
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A20-P730
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1%
- Sensitivity
- <20 pg/mL
- Function
- Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 80535 MW
- Specificity
- Natural and recombinant mouse total MMP-9
- Anticoagulant
- Heparin
- Gene Name Synonym
- Matrix metalloproteinase-9
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Protein Name
- Matrix metalloproteinase-9
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
