- Catalog number
- EK0474
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 38 39 39 39 1.08 2.7% Sample 2 173 159 176 168 169 6.44 3.8% Sample 3 464 416 419 479 444 27.53 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 38 39 39 39 1.08 2.7% Sample 1 41 38 39 39 39 1.08 2.7% Sample 2 173 159 176 168 169 6.44 3.8% Sample 2 173 159 176 168 169 6.44 3.8% Sample 3 464 416 419 479 444 27.53 6.2% Sample 3 464 416 419 479 444 27.53 6.2%
- Synonyms
- Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; Ntf3; Ntf-3
- Gene Name
- NTF3
- Gene ID
- 81737
- UniProt
- P18280
- Reactivity
- Rat
- Cross Reactivity
- There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y139-T257
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Brain and peripheral tissues.
- Applications
- ELISA
- Field of Research
- Growth and Development, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5%
- Sensitivity
- <2 pg/mL
- Function
- Seems to promote the survival of visceral and proprioceptive sensory neurons.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 29645 MW
- Specificity
- Natural and recombinant rat Neurotrophin-3
- Gene Name Synonym
- Neurotrophin-3
- Subcellular Location
- Secreted.
- Protein Name
- Neurotrophin-3
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
