- Catalog number
- EK0481
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 157 157 146 148 9.88 6.6% Sample 2 800 766 743 710 754 32.84 4.3% Sample 3 3535 3949 3581 3584 3662 166.69 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 157 157 146 148 9.88 6.6% Sample 1 133 157 157 146 148 9.88 6.6% Sample 2 800 766 743 710 754 32.84 4.3% Sample 2 800 766 743 710 754 32.84 4.3% Sample 3 3535 3949 3581 3584 3662 166.69 4.5% Sample 3 3535 3949 3581 3584 3662 166.69 4.5%
- Synonyms
- Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; Tnfrsf11b; Ocif, Opg
- Gene Name
- TNFRSF11B
- Gene ID
- 18383
- UniProt
- O08712
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E22-L401
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Metabolism, Obesity, Signal Transduction, Structures, TNF Superfamily, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.39 55.39 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.39 55.39 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.39 55.39 225.86 Standard deviation 5.85 54.4 187.35 7.39 55.39 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2%
- Sensitivity
- <5 pg/mL
- Function
- Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 45923 MW
- Specificity
- Natural and recombinant mouse OPG
- Anticoagulant
- EDTA
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 11B
- Subcellular Location
- Secreted.
- Sequence Similarities
- Contains 2 death domains.
- Protein Name
- Tumor necrosis factor receptor superfamily member 11B
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.071 0.138 0.275 0.536 0.92 1.532 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.071 0.138 0.275 0.536 0.92 1.532 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.071 0.138 0.275 0.536 0.92 1.532 2.158 O.D. 0.014 0.071 0.138 0.275 0.536 0.92 1.532 2.158
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
