- Catalog number
- EK0482
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1) . Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 305 309 272 322 302 18.42 6% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 3 4985 4178 4476 4405 4511 294.96 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 305 309 272 322 302 18.42 6% Sample 1 305 309 272 322 302 18.42 6% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 3 4985 4178 4476 4405 4511 294.96 6.5% Sample 3 4985 4178 4476 4405 4511 294.96 6.5%
- Synonyms
- Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP, OPN; PSEC0156
- Gene Name
- SPP1
- Gene ID
- 6696
- UniProt
- P10451
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: I17-N300
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Bone. Found in plasma.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1%
- Sensitivity
- <10 pg/mL
- Function
- Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 35423 MW
- Specificity
- Natural and recombinant human OPN
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Osteopontin
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the osteopontin family.
- Protein Name
- Osteopontin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
