- Catalog number
- EK0502
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) . A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 290 284 281 6.53 2.3% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 3 4329 4374 4283 4168 4288 76.65 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 290 284 281 6.53 2.3% Sample 1 272 280 290 284 281 6.53 2.3% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 3 4329 4374 4283 4168 4288 76.65 1.7% Sample 3 4329 4374 4283 4168 4288 76.65 1.7%
- Synonyms
- E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1
- Gene Name
- SELE
- Gene ID
- 20339
- UniProt
- Q00690
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: W22-P557
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cofactors, Vitamins/Minerals, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Tumor Suppressors, Vitamins/Minerals
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 66750 MW
- Specificity
- Natural and recombinant mouse sE-Selectin
- Anticoagulant
- Heparin or citrate
- Gene Name Synonym
- E-selectin
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the selectin/LECAM family.
- Protein Name
- E-selectin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.069 0.101 0.173 0.323 0.577 1.092 1.856 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.069 0.101 0.173 0.323 0.577 1.092 1.856 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.069 0.101 0.173 0.323 0.577 1.092 1.856 O.D. 0.027 0.069 0.101 0.173 0.323 0.577 1.092 1.856
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
