- Catalog number
- EK0511
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 31 35 32 1.65 5.1% Sample 2 230 219 227 246 230 9.81 4.2% Sample 3 448 498 470 454 467 19.35 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 31 35 32 1.65 5.1% Sample 1 31 33 31 35 32 1.65 5.1% Sample 2 230 219 227 246 230 9.81 4.2% Sample 2 230 219 227 246 230 9.81 4.2% Sample 3 448 498 470 454 467 19.35 4.1% Sample 3 448 498 470 454 467 19.35 4.1%
- Synonyms
- Protransforming growth factor alpha; Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1; TGFA
- Gene Name
- TGFA
- Gene ID
- 7039
- UniProt
- P01135
- Reactivity
- Human
- Cross Reactivity
- There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V40-A89
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9%
- Sensitivity
- <1 pg/mL
- Function
- TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17006 MW
- Specificity
- Natural and recombinant human TGF alpha
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Protransforming growth factor alpha
- Subcellular Location
- Transforming growth factor alpha: Secreted, extracellular space.
- Protein Name
- Protransforming growth factor alpha
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
