- Catalog number
- EK0514
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 31 29 32 29 1.92 6.6% Sample 2 161 143 162 145 152 8.78 5.7% Sample 3 431 413 424 455 430 15.4 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 31 29 32 29 1.92 6.6% Sample 1 27 31 29 32 29 1.92 6.6% Sample 2 161 143 162 145 152 8.78 5.7% Sample 2 161 143 162 145 152 8.78 5.7% Sample 3 431 413 424 455 430 15.4 3.5% Sample 3 431 413 424 455 430 15.4 3.5%
- Synonyms
- Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP; Tgfb1
- Gene Name
- TGFB1
- Gene ID
- 59086
- UniProt
- P17246
- Reactivity
- Rat
- Cross Reactivity
- There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1%.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A279-S390
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Abundant in the bone matrix. .
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Co-Factors, Cofactors, Vitamins/Minerals, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (EDTA) and urine.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 161 431 28 156 422 Standard deviation 1.59 9.82 23.7 1.73 9.98 26.16 CV (%) 5.5% 6.1% 5.5% 6.2% 6.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 161 431 28 156 422 Standard deviation 1.59 9.82 23.7 1.73 9.98 26.16 CV (%) 5.5% 6.1% 5.5% 6.2% 6.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 161 431 28 156 422 Mean (pg/ml) 27 161 431 28 156 422 Standard deviation 1.59 9.82 23.7 1.73 9.98 26.16 Standard deviation 1.59 9.82 23.7 1.73 9.98 26.16 CV (%) 5.5% 6.1% 5.5% 6.2% 6.4% 6.2% CV (%) 5.5% 6.1% 5.5% 6.2% 6.4% 6.2%
- Sensitivity
- <1 pg/mL
- Function
- Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 44329 MW
- Specificity
- Natural and recombinant rat TGF beta 1
- Anticoagulant
- EDTA
- Gene Name Synonym
- Transforming growth factor beta-1
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Protein Name
- Transforming growth factor beta-1
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.048 0.119 0.177 0.299 0.492 0.857 1.463 2.223 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.048 0.119 0.177 0.299 0.492 0.857 1.463 2.223 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.048 0.119 0.177 0.299 0.492 0.857 1.463 2.223 O.D. 0.048 0.119 0.177 0.299 0.492 0.857 1.463 2.223
- Activating Reagent
- Solution A: 2Solution B: 22
- Sample Activation Procedure
- Activate the sample Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (EDTA) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGFβ 1. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (EDTA) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGFβ 1. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
