- Catalog number
- EK0517
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF) . TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 87 100 93 93 4.63 4.9% Sample 2 307 267 309 301 296 17 5.7% Sample 3 851 915 849 807 855 38.58 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 87 100 93 93 4.63 4.9% Sample 1 92 87 100 93 93 4.63 4.9% Sample 2 307 267 309 301 296 17 5.7% Sample 2 307 267 309 301 296 17 5.7% Sample 3 851 915 849 807 855 38.58 4.5% Sample 3 851 915 849 807 855 38.58 4.5%
- Synonyms
- Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Thpo
- Gene Name
- TPO
- Gene ID
- 21832
- UniProt
- P40226
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S22-T356
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found mainly in the liver, kidney and skeletal muscle.
- Applications
- ELISA
- Field of Research
- Zinc Finger
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6%
- Sensitivity
- <30 pg/mL
- Function
- Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 37836 MW
- Specificity
- Natural and recombinant mouse TPO
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Thrombopoietin
- Subcellular Location
- Secreted.
- Protein Name
- Thrombopoietin
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
