- Catalog number
- EK0522
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Sample 3 4399 5066 4506 5016 4746 297.19 6.2%
- Synonyms
- Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2; TIMP2
- Gene Name
- TIMP2
- Gene ID
- 7077
- UniProt
- P16035
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: C27-P220
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3%
- Sensitivity
- <2 pg/mL
- Function
- Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24399 MW
- Specificity
- Natural and recombinant human TIMP-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Metalloproteinase inhibitor 2
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Protein Name
- Metalloproteinase inhibitor 2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
