- Catalog number
- EK0523
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Sample 3 4383 3655 4220 4320 4144 288.52 6.9%
- Synonyms
- Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3; TIMP3
- Gene Name
- TIMP3
- Gene ID
- 7078
- UniProt
- P35625
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C24-P211
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Signal Transduction, Visual System
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1%
- Sensitivity
- <2 pg/mL
- Function
- Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24145 MW
- Specificity
- Natural and recombinant human TIMP-3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Metalloproteinase inhibitor 3
- Subcellular Location
- Secreted, extracellular space, extracellular matrix.
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Protein Name
- Metalloproteinase inhibitor 3
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
