- Catalog number
- EK0524
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 131 119 122 123 4.43 3.6% Sample 2 589 618 605 599 602 10.49 1.7% Sample 3 2056 2211 2029 2343 2159 126.55 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 131 119 122 123 4.43 3.6% Sample 1 123 131 119 122 123 4.43 3.6% Sample 2 589 618 605 599 602 10.49 1.7% Sample 2 589 618 605 599 602 10.49 1.7% Sample 3 2056 2211 2029 2343 2159 126.55 5.8% Sample 3 2056 2211 2029 2343 2159 126.55 5.8%
- Synonyms
- Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4; TIMP4
- Gene Name
- TIMP4
- Gene ID
- 7079
- UniProt
- Q99727
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C30-P224
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Abundant in heart and present at low levels in many other tissues.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25503 MW
- Specificity
- Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Metalloproteinase inhibitor 4
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Protein Name
- Metalloproteinase inhibitor 4
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.001 0.071 0.15 0.312 0.594 0.989 1.553 2.026 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.001 0.071 0.15 0.312 0.594 0.989 1.553 2.026 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.001 0.071 0.15 0.312 0.594 0.989 1.553 2.026 O.D. 0.001 0.071 0.15 0.312 0.594 0.989 1.553 2.026
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
