- Catalog number
- EK0526
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 14 14 14 14 0 0% Sample 2 96 93 92 101 95 3.5 3.6% Sample 3 280 258 274 306 279 17.28 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 14 14 14 14 0 0% Sample 1 14 14 14 14 14 0 0% Sample 2 96 93 92 101 95 3.5 3.6% Sample 2 96 93 92 101 95 3.5 3.6% Sample 3 280 258 274 306 279 17.28 6.1% Sample 3 280 258 274 306 279 17.28 6.1%
- Synonyms
- Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2
- Gene Name
- TNF
- Gene ID
- 24835
- UniProt
- P16599
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L80-L235
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Metabolism, Signal Transduction, Immunology, Cancer, Atherosclerosis, Cardiovascular, Growth Factors/Hormones, Growth Factors, Innate Immunity, Vascular Inflammation, Cytokines, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 96 280 13 98 306 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 96 280 13 98 306 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 14 96 280 13 98 306 Mean (pg/ml) 14 96 280 13 98 306 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1% CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1%
- Sensitivity
- <1 pg/mL
- Function
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25806 MW
- Specificity
- Natural and recombinant rat TNF alpha
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor
- Subcellular Location
- Cell membrane ; Single-pass type II membrane protein .
- Protein Name
- Tumor necrosis factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
