- Catalog number
- EK0527
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Human TNF
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% Sample 3 299 294 293 285 292 5.01 1.7%
- Synonyms
- Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2
- Gene Name
- TNF
- Gene ID
- 21926
- UniProt
- P06804
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L80-L235
- Capture Antibody
- Monoclonal antibody from hamster
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Metabolism, Signal Transduction, Immunology, Cancer, Atherosclerosis, Cardiovascular, Growth Factors/Hormones, Growth Factors, Innate Immunity, Vascular Inflammation, Cytokines, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8%
- Sensitivity
- <1 pg/mL
- Function
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25896 MW
- Specificity
- Natural and recombinant Mouse TNF alpha
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor
- Subcellular Location
- Cell membrane; Single-pass type II membrane protein.
- Protein Name
- Tumor necrosis factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
