- Catalog number
- EK0530
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II (TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 17 16 16 0.43 2.6% Sample 2 58 61 58 56 58 1.78 3% Sample 3 235 274 227 275 252 21.93 8.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 17 16 16 0.43 2.6% Sample 1 16 16 17 16 16 0.43 2.6% Sample 2 58 61 58 56 58 1.78 3% Sample 2 58 61 58 56 58 1.78 3% Sample 3 235 274 227 275 252 21.93 8.7% Sample 3 235 274 227 275 252 21.93 8.7%
- Synonyms
- Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept; Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding protein 2; TBP-2; TBPII; TNFRSF1B; TNFBR, TNFR2
- Gene Name
- TNFRSF1B
- Gene ID
- 7133
- UniProt
- P20333
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L23-D257
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Growth Factors/Hormones, Receptors, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6%
- Sensitivity
- <2 pg/mL
- Function
- Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 48291 MW
- Specificity
- Natural and recombinant human sTNFsRII
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 1B
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 4 TNFR-Cys repeats.
- Protein Name
- Tumor necrosis factor receptor superfamily member 1B
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
