- Catalog number
- EK0537
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 273 296 273 288 15.79 5.4% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 3 5287 5539 5884 6073 5695 303.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 273 296 273 288 15.79 5.4% Sample 1 310 273 296 273 288 15.79 5.4% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 3 5287 5539 5884 6073 5695 303.89 5.3% Sample 3 5287 5539 5884 6073 5695 303.89 5.3%
- Synonyms
- Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1; L1CAM
- Gene Name
- VCAM1
- Gene ID
- 7412
- UniProt
- P19320
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: F25-E698
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Mesenchymal Stem Cells, Neural Signal Transduction, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6%
- Sensitivity
- <10 pg/mL
- Function
- Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 81276 MW
- Specificity
- Natural and recombinant human VCAM-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Vascular cell adhesion protein 1
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
- Protein Name
- Vascular cell adhesion protein 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
