Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine®

About this product

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ANGPT1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0559

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 209 251 225 234 18.15 7.7% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 3 5201 4542 5134 5110 4996 264.65 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 209 251 225 234 18.15 7.7% Sample 1 252 209 251 225 234 18.15 7.7% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 3 5201 4542 5134 5110 4996 264.65 5.2% Sample 3 5201 4542 5134 5110 4996 264.65 5.2%

Synonyms

Angiopoietin-1; ANG-1; ANGPT1; KIAA0003

Gene Name

ANGPT1

Gene ID

284

UniProt

Q15389

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H16-F498

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Growth Factors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1375 5201 272 1440 5518 Standard deviation 15.37 90.75 358.86 17.68 102.24 452.47 CV (%) 6.1% 6.6% 6.9% 6.5% 7.1% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1375 5201 272 1440 5518 Standard deviation 15.37 90.75 358.86 17.68 102.24 452.47 CV (%) 6.1% 6.6% 6.9% 6.5% 7.1% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 252 1375 5201 272 1440 5518 Mean (pg/ml) 252 1375 5201 272 1440 5518 Standard deviation 15.37 90.75 358.86 17.68 102.24 452.47 Standard deviation 15.37 90.75 358.86 17.68 102.24 452.47 CV (%) 6.1% 6.6% 6.9% 6.5% 7.1% 8.2% CV (%) 6.1% 6.6% 6.9% 6.5% 7.1% 8.2%

Sensitivity

<10 pg/mL

Function

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

57513 MW

Specificity

Natural and recombinant human Angiopoietin-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin-1

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 fibrinogen C-terminal domain.

Protein Name

Angiopoietin-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.165 0.231 0.367 0.624 1.03 1.641 2.04 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.165 0.231 0.367 0.624 1.03 1.641 2.04 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.165 0.231 0.367 0.624 1.03 1.641 2.04 O.D. 0.066 0.165 0.231 0.367 0.624 1.03 1.641 2.04

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.