- Catalog number
- EK0561
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+) -dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 266 300 267 274 14.73 5.3% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 3 4789 4996 5212 5707 5176 341.11 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 266 300 267 274 14.73 5.3% Sample 1 265 266 300 267 274 14.73 5.3% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 3 4789 4996 5212 5707 5176 341.11 6.5% Sample 3 4789 4996 5212 5707 5176 341.11 6.5%
- Synonyms
- Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; CDH1; CDHE, UVO
- Gene Name
- CDH1
- Gene ID
- 999
- UniProt
- P12830
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D155-I707
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Non-neural epithelial tissues.
- Applications
- ELISA
- Field of Research
- Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin), saliva and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1264 4789 253 1258 4691 Standard deviation 15.37 58.14 324.65 16.69 61.64 318.98 CV (%) 5.8% 4.6% 6.8% 6.6% 4.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1264 4789 253 1258 4691 Standard deviation 15.37 58.14 324.65 16.69 61.64 318.98 CV (%) 5.8% 4.6% 6.8% 6.6% 4.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 265 1264 4789 253 1258 4691 Mean (pg/ml) 265 1264 4789 253 1258 4691 Standard deviation 15.37 58.14 324.65 16.69 61.64 318.98 Standard deviation 15.37 58.14 324.65 16.69 61.64 318.98 CV (%) 5.8% 4.6% 6.8% 6.6% 4.9% 6.8% CV (%) 5.8% 4.6% 6.8% 6.6% 4.9% 6.8%
- Sensitivity
- <15 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 97456 MW
- Specificity
- Natural and recombinant human E-Cadherin
- Anticoagulant
- Heparin
- Gene Name Synonym
- Cadherin-1
- Subcellular Location
- Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
- Sequence Similarities
- Contains 5 cadherin domains.
- Protein Name
- Cadherin-1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.086 0.122 0.192 0.325 0.547 1.043 1.884 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.086 0.122 0.192 0.325 0.547 1.043 1.884 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.086 0.122 0.192 0.325 0.547 1.043 1.884 O.D. 0.048 0.086 0.122 0.192 0.325 0.547 1.043 1.884
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
