- Catalog number
- EK0562
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+) -dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Sample 3 5631 5520 5145 4973 5317 268.19 5%
- Synonyms
- Cadherin-1; ARC-1; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Cdh1
- Gene Name
- CDH1
- Gene ID
- 12550
- UniProt
- P09803
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D157-V709
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Non-neural epithelial tissues.
- Applications
- ELISA
- Field of Research
- Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3%
- Sensitivity
- <15 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 98256 MW
- Specificity
- Natural and recombinant mouse E-Cadherin
- Anticoagulant
- Heparin
- Gene Name Synonym
- Cadherin-1
- Subcellular Location
- Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity) . .
- Protein Name
- Cadherin-1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.072 0.104 0.162 0.292 0.58 1.126 2.069 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.072 0.104 0.162 0.292 0.58 1.126 2.069 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.072 0.104 0.162 0.292 0.58 1.126 2.069 O.D. 0.044 0.072 0.104 0.162 0.292 0.58 1.126 2.069
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
