- Catalog number
- EK0565
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 28 28 28 0.86 3% Sample 2 196 177 181 179 183 7.49 4% Sample 3 432 458 433 415 434 15.33 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 28 28 28 0.86 3% Sample 1 30 28 28 28 28 0.86 3% Sample 2 196 177 181 179 183 7.49 4% Sample 2 196 177 181 179 183 7.49 4% Sample 3 432 458 433 415 434 15.33 3.5% Sample 3 432 458 433 415 434 15.33 3.5%
- Synonyms
- C-C motif chemokine 1; Small-inducible cytokine A1; T lymphocyte-secreted protein I-309; CCL1; SCYA1
- Gene Name
- CCL1
- Gene ID
- 6346
- UniProt
- P22362
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: K24-K96
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9%
- Sensitivity
- <4 pg/mL
- Function
- Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10992 MW
- Specificity
- Natural and recombinant human CCL1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 1
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Protein Name
- C-C motif chemokine 1
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
