- Catalog number
- EK0566
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 15 16 16 16 0.7 4.3% Sample 2 81 79 85 79 81 2.44 3% Sample 3 291 314 273 296 293 14.6 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 15 16 16 16 0.7 4.3% Sample 1 17 15 16 16 16 0.7 4.3% Sample 2 81 79 85 79 81 2.44 3% Sample 2 81 79 85 79 81 2.44 3% Sample 3 291 314 273 296 293 14.6 4.9% Sample 3 291 314 273 296 293 14.6 4.9%
- Synonyms
- C-C motif chemokine 1; P500; SIS-epsilon; Small-inducible cytokine A1; T-cell activation protein 3; TCA-3; TCA3; Ccl1; Scya1, Tca3
- Gene Name
- CCL1
- Gene ID
- 20290
- UniProt
- P10146
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: K24-C92
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 291 16 78 277 Standard deviation 0.9 5.5 18.91 1.02 6.78 20.22 CV (%) 5.3% 6.8% 6.5% 6.4% 8.7% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 291 16 78 277 Standard deviation 0.9 5.5 18.91 1.02 6.78 20.22 CV (%) 5.3% 6.8% 6.5% 6.4% 8.7% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 291 16 78 277 Mean (pg/ml) 17 81 291 16 78 277 Standard deviation 0.9 5.5 18.91 1.02 6.78 20.22 Standard deviation 0.9 5.5 18.91 1.02 6.78 20.22 CV (%) 5.3% 6.8% 6.5% 6.4% 8.7% 7.3% CV (%) 5.3% 6.8% 6.5% 6.4% 8.7% 7.3%
- Sensitivity
- <1 pg/mL
- Function
- Cytokine that is chemotactic for neutrophils.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10276 MW
- Specificity
- Natural and recombinant mouse CCL1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 1
- Protein Name
- C-C motif chemokine 1
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.026 0.108 0.168 0.321 0.627 1.043 1.676 2.23 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.026 0.108 0.168 0.321 0.627 1.043 1.676 2.23 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.026 0.108 0.168 0.321 0.627 1.043 1.676 2.23 O.D. 0.026 0.108 0.168 0.321 0.627 1.043 1.676 2.23
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
