- Catalog number
- EK0570
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 35 38 39 38 1.87 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 3 414 393 421 433 415 14.53 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 35 38 39 38 1.87 4.9% Sample 1 40 35 38 39 38 1.87 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 3 414 393 421 433 415 14.53 3.5% Sample 3 414 393 421 433 415 14.53 3.5%
- Synonyms
- Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; CD30; Tnfrsf8
- Gene Name
- TNFRSF8
- Gene ID
- 21941
- UniProt
- Q60846
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: F19-T281
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected in thymus and in activated splenocytes.
- Applications
- ELISA
- Field of Research
- Cancer, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Receptors, TNF Superfamily, Tumor Biomarkers, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 53216 MW
- Specificity
- Natural and recombinant mouse CD30
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 8
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Tumor necrosis factor receptor superfamily member 8
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
