- Catalog number
- EK0576
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 38 39 39 38 0.82 2.1% Sample 2 220 203 230 223 219 9.92 4.5% Sample 3 498 423 499 448 467 32.71 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 38 39 39 38 0.82 2.1% Sample 1 37 38 39 39 38 0.82 2.1% Sample 2 220 203 230 223 219 9.92 4.5% Sample 2 220 203 230 223 219 9.92 4.5% Sample 3 498 423 499 448 467 32.71 7% Sample 3 498 423 499 448 467 32.71 7%
- Synonyms
- C-C motif chemokine 24; Eosinophil chemotactic protein 2; Eotaxin-2; Small-inducible cytokine A24; Ccl24; Scya24
- Gene Name
- CCL24
- Gene ID
- 56221
- UniProt
- Q9JKC0
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V27-V119
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis. .
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 12871 MW
- Specificity
- Natural and recombinant mouse CCL24
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 24
- Subcellular Location
- Secreted.
- Protein Name
- C-C motif chemokine 24
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
