- Catalog number
- EK0578
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 31 29 29 0.86 2.9% Sample 2 192 199 200 197 197 3.08 1.5% Sample 3 394 427 443 440 426 19.42 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 31 29 29 0.86 2.9% Sample 1 29 29 31 29 29 0.86 2.9% Sample 2 192 199 200 197 197 3.08 1.5% Sample 2 192 199 200 197 197 3.08 1.5% Sample 3 394 427 443 440 426 19.42 4.5% Sample 3 394 427 443 440 426 19.42 4.5%
- Synonyms
- Growth hormone 1 isoform 1 ; HCG1749481, isoform CRA_s ; GH1 ; hCG_1749481
- Gene Name
- GH1
- Gene ID
- 2688
- UniProt
- P01241
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: F27-F217
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Developmental Biology, Growth Factors/Hormones, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24847 MW
- Specificity
- Natural and recombinant human GH1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Growth hormone 1
- Subcellular Location
- Secreted .
- Protein Name
- Somatotropin
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
