- Catalog number
- EK0592
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin (IL) -18
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 30 28 29 0.7 2.4% Sample 2 166 149 165 152 158 7.58 4.7% Sample 3 511 489 520 499 504 11.75 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 30 28 29 0.7 2.4% Sample 1 29 29 30 28 29 0.7 2.4% Sample 2 166 149 165 152 158 7.58 4.7% Sample 2 166 149 165 152 158 7.58 4.7% Sample 3 511 489 520 499 504 11.75 2.3% Sample 3 511 489 520 499 504 11.75 2.3%
- Synonyms
- Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Il18; Igif
- Gene Name
- IL18
- Gene ID
- 29197
- UniProt
- P97636
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: H37-S194
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6%
- Sensitivity
- <1 pg/mL
- Function
- Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22303 MW
- Specificity
- Natural and recombinant rat IL-18
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-18
- Subcellular Location
- Secreted.
- Protein Name
- Interleukin-18
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
