- Catalog number
- EK0593
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 266 323 299 291 21.68 7.4% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 3 4706 4695 5058 4926 4846 153.08 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 266 323 299 291 21.68 7.4% Sample 1 278 266 323 299 291 21.68 7.4% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 3 4706 4695 5058 4926 4846 153.08 3.1% Sample 3 4706 4695 5058 4926 4846 153.08 3.1%
- Synonyms
- Prolactin; PRL; PRL
- Gene Name
- PRL
- Gene ID
- 5617
- UniProt
- P01236
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L29-C227
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Growth Factors/Hormones, Lipid Metabolism, Metabolism, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4%
- Sensitivity
- <10 pg/mL
- Function
- Prolactin acts primarily on the mammary gland by promoting lactation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25876 MW
- Specificity
- Natural and recombinant human Prolactin
- Anticoagulant
- Heparin or citrate
- Gene Name Synonym
- Prolactin
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the somatotropin/prolactin family.
- Protein Name
- Prolactin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
