- Catalog number
- EK0594
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 250 249 260 251 5.02 2% Sample 2 1548 1485 1544 1578 1538 33.69 2.1% Sample 3 4124 4396 4363 4724 4401 213.61 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 250 249 260 251 5.02 2% Sample 1 247 250 249 260 251 5.02 2% Sample 2 1548 1485 1544 1578 1538 33.69 2.1% Sample 2 1548 1485 1544 1578 1538 33.69 2.1% Sample 3 4124 4396 4363 4724 4401 213.61 4.8% Sample 3 4124 4396 4363 4724 4401 213.61 4.8%
- Synonyms
- Prolactin; PRL; Prl
- Gene Name
- PRL
- Gene ID
- 19109
- UniProt
- P06879
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L32-C228
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Growth Factors/Hormones, Lipid Metabolism, Metabolism, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1548 4124 254 1402 4314 Standard deviation 13.83 68.11 206.2 15.74 82.71 228.64 CV (%) 4.4%% 4.4% 5% 6.2% 5.9% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1548 4124 254 1402 4314 Standard deviation 13.83 68.11 206.2 15.74 82.71 228.64 CV (%) 4.4%% 4.4% 5% 6.2% 5.9% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1548 4124 254 1402 4314 Mean (pg/ml) 247 1548 4124 254 1402 4314 Standard deviation 13.83 68.11 206.2 15.74 82.71 228.64 Standard deviation 13.83 68.11 206.2 15.74 82.71 228.64 CV (%) 4.4%% 4.4% 5% 6.2% 5.9% 5.3% CV (%) 4.4%% 4.4% 5% 6.2% 5.9% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Prolactin acts primarily on the mammary gland by promoting lactation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25496 MW
- Specificity
- Natural and recombinant mouse Prolactin
- Anticoagulant
- Heparin or citrate
- Gene Name Synonym
- Prolactin
- Subcellular Location
- Secreted.
- Protein Name
- Prolactin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
