- Catalog number
- EK0596
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 567 603 628 571 592 24.91 4.2% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 3 7572 7510 8167 7413 7665 295.03 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 567 603 628 571 592 24.91 4.2% Sample 1 567 603 628 571 592 24.91 4.2% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 3 7572 7510 8167 7413 7665 295.03 3.8% Sample 3 7572 7510 8167 7413 7665 295.03 3.8%
- Synonyms
- Adiponectin;30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adipoq; Acdc, Acrp30, Apm1
- Gene Name
- ADIPOQ
- Gene ID
- 11450
- UniProt
- Q60994
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E18-N244
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Synthesized exclusively by adipocytes and secreted into plasma.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Diabetes, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Lipids/Lipoproteins, Mesenchymal Stem Cells, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Response To Hypoxia, Signal Transduction, Stem Cells
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4%
- Sensitivity
- <10 pg/mL
- Function
- Important adipokine involved in the control of fat metabolism and insulin sensitivity, with anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26809 MW
- Specificity
- Natural and recombinant full length mouse Adiponectin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Adiponectin
- Subcellular Location
- Secreted.
- Sequence Similarities
- Contains 1 C1q domain.
- Protein Name
- Adiponectin
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.092 0.164 0.304 0.589 0.966 1.517 2.038 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.092 0.164 0.304 0.589 0.966 1.517 2.038 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.092 0.164 0.304 0.589 0.966 1.517 2.038 O.D. 0.035 0.092 0.164 0.304 0.589 0.966 1.517 2.038
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
