- Catalog number
- EK0642
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 217 210 226 13.79 6.1% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 3 6045 5875 6096 5562 5894 208.67 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 217 210 226 13.79 6.1% Sample 1 234 245 217 210 226 13.79 6.1% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 3 6045 5875 6096 5562 5894 208.67 3.5% Sample 3 6045 5875 6096 5562 5894 208.67 3.5%
- Synonyms
- Thrombospondin-2; THBS2; TSP2
- Gene Name
- THBS2
- Gene ID
- 7058
- UniProt
- P35442
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G19-I1172
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- High expression in invertebral disk tissue. .
- Applications
- ELISA
- Standard
- 40 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
- Detection Range
- 625 pg/mL - 40,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7%
- Sensitivity
- <10 pg/mL
- Function
- Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 129991 MW
- Specificity
- Natural and recombinant human TSP2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Thrombospondin-2
- Sequence Similarities
- Belongs to the thrombospondin family.
- Protein Name
- Thrombospondin-2
- Sample Data
- Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
