- Catalog number
- EK0662
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 83 96 86 5.59 6.5% Sample 2 288 282 296 300 291 6.98 2.3% Sample 3 757 861 852 799 817 42.08 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 83 96 86 5.59 6.5% Sample 1 82 85 83 96 86 5.59 6.5% Sample 2 288 282 296 300 291 6.98 2.3% Sample 2 288 282 296 300 291 6.98 2.3% Sample 3 757 861 852 799 817 42.08 5.1% Sample 3 757 861 852 799 817 42.08 5.1%
- Synonyms
- Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; Tnfrsf17; Bcm, Bcma
- Gene Name
- TNFRSF17
- Gene ID
- 21935
- UniProt
- O88472
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M1-T49
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected in spleen, thymus, bone marrow and heart, and at lower levels in kidney and lung.
- Applications
- ELISA
- Field of Research
- Antiviral Signaling, Cancer, Growth Factors, Growth Factors/Hormones, Immune System Diseases, Immunology, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20442 MW
- Specificity
- Natural and recombinant mouse TNFRSF17
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 17
- Subcellular Location
- Membrane ; Single-pass type III membrane protein .
- Protein Name
- Tumor necrosis factor receptor superfamily member 17
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
