- Catalog number
- EK0664
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 90 80 84 3.76 4.4% Sample 2 293 335 318 295 310 17.34 5.5% Sample 3 1221 1161 1374 1312 1267 81.89 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 90 80 84 3.76 4.4% Sample 1 82 85 90 80 84 3.76 4.4% Sample 2 293 335 318 295 310 17.34 5.5% Sample 2 293 335 318 295 310 17.34 5.5% Sample 3 1221 1161 1374 1312 1267 81.89 6.4% Sample 3 1221 1161 1374 1312 1267 81.89 6.4%
- Synonyms
- Tumor necrosis factor (Ligand) superfamily, member 13b ; Tumor necrosis factor (Ligand) superfamily, member 13b, isoform CRA_d ; Tnfsf13b ; mCG_3400
- Gene Name
- TNFSF13B
- Gene ID
- 24099
- UniProt
- Q3KP92
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A127-L309
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Non-CD, Signal Transduction, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 34192 MW
- Specificity
- Natural and recombinant mouse BAFF
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor (ligand) superfamily, member 13b
- Protein Name
- Tumor necrosis factor ligand superfamily member 13B
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
