- Catalog number
- EK0668
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- P-Cadherin, also known as Cadherin-3 (CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. Cells expressing CDH3 also adhered to one another more tightly than the parental cell line.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 198 204 177 182 190 11.09 5.8% Sample 2 571 533 614 577 573 28.71 5% Sample 3 1283 1280 1261 1323 1286 22.56 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 198 204 177 182 190 11.09 5.8% Sample 1 198 204 177 182 190 11.09 5.8% Sample 2 571 533 614 577 573 28.71 5% Sample 2 571 533 614 577 573 28.71 5% Sample 3 1283 1280 1261 1323 1286 22.56 1.7% Sample 3 1283 1280 1261 1323 1286 22.56 1.7%
- Synonyms
- Cadherin-3; Placental cadherin; P-cadherin; Cdh3; Cdhp
- Gene Name
- CDH3
- Gene ID
- 12560
- UniProt
- P10287
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E100-G647
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cadherins, Cell Adhesion, Cytoskeleton/ECM, Neuroscience, Sensory System, Signal Transduction, Visual System
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 198 571 1283 216 594 1188 Standard deviation 7.92 34.5%7 57.73 12.52 43.95 73.65 CV (%) 4% 7.4%% 4.5% 5.8% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 198 571 1283 216 594 1188 Standard deviation 7.92 34.5%7 57.73 12.52 43.95 73.65 CV (%) 4% 7.4%% 4.5% 5.8% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 198 571 1283 216 594 1188 Mean (pg/ml) 198 571 1283 216 594 1188 Standard deviation 7.92 34.5%7 57.73 12.52 43.95 73.65 Standard deviation 7.92 34.5%7 57.73 12.52 43.95 73.65 CV (%) 4% 7.4%% 4.5% 5.8% 7.4% 6.2% CV (%) 4% 7.4%% 4.5% 5.8% 7.4% 6.2%
- Sensitivity
- <10 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 90612 MW
- Specificity
- Oligomeric forms of natural and recombinant mouse P-Cadherin
- Gene Name Synonym
- Cadherin-3
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Cadherin-3
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.037 0.106 0.171 0.259 0.609 0.796 1.452 2.004 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.037 0.106 0.171 0.259 0.609 0.796 1.452 2.004 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.037 0.106 0.171 0.259 0.609 0.796 1.452 2.004 O.D. 0.037 0.106 0.171 0.259 0.609 0.796 1.452 2.004
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
