- Catalog number
- EK0672
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3%
- Synonyms
- Cathepsin D;3.4.23.5; Cathepsin D light chain; Cathepsin D heavy chain; CTSD; CPSD
- Gene Name
- CTSD
- Gene ID
- 1509
- UniProt
- P07339
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L21-L412
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in the aorta extrcellular space (at protein level) . .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3%
- Sensitivity
- <10 pg/mL
- Function
- Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 44552 MW
- Specificity
- Natural and recombinant human Cathepsin D
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cathepsin D
- Subcellular Location
- Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380) . .
- Sequence Similarities
- Belongs to the peptidase A1 family.
- Protein Name
- Cathepsin D
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
