Mouse Cathepsin D ELISA Kit PicoKine®

About this product

Mouse Cathepsin D ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0673

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 844 838 787 752 805 37.89 4.7% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 3 7768 8873 9192 9277 8777 601.98 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 844 838 787 752 805 37.89 4.7% Sample 1 844 838 787 752 805 37.89 4.7% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 3 7768 8873 9192 9277 8777 601.98 6.8% Sample 3 7768 8873 9192 9277 8777 601.98 6.8%

Synonyms

Cathepsin D;3.4.23.5; CTSD

Gene Name

CTSD

Gene ID

13033

UniProt

Q3UCD9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I21-L410

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4%

Sensitivity

<10 pg/mL

Function

Acid protease active in intracellular protein breakdown.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44954 MW

Specificity

Natural and recombinant mouse Cathepsin D

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin D

Subcellular Location

Lysosome. Melanosome . Secreted, extracellular space .

Protein Name

Cathepsin D

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.