- Catalog number
- EK0682
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Sample 3 412 423 456 402 423 20.31 4.8%
- Synonyms
- Carbonic anhydrase 9;4.2.1.1; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1; CA9; G250, MN
- Gene Name
- CA9
- Gene ID
- 768
- UniProt
- Q16790
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: P59-D414
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Immunology, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7%
- Sensitivity
- <10 pg/mL
- Function
- Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 49698 MW
- Specificity
- Natural and recombinant human CA9
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Carbonic anhydrase 9
- Subcellular Location
- Nucleus . Nucleus, nucleolus . Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus.
- Protein Name
- Carbonic anhydrase 9
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
