- Catalog number
- EK0683
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Sample 3 459 441 412 484 449 26.25 5.8%
- Synonyms
- C-C motif chemokine 7; Intercrine/chemokine MARC; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Protein FIC; Small-inducible cytokine A7; Ccl7; Fic, Mcp3, Scya7
- Gene Name
- CCL7
- Gene ID
- 20306
- UniProt
- Q03366
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Q24-P97
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6%
- Sensitivity
- <1 pg/mL
- Function
- Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10999 MW
- Specificity
- Natural and recombinant mouse CCL7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 7
- Subcellular Location
- Secreted.
- Protein Name
- C-C motif chemokine 7
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
