- Catalog number
- EK0684
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 58 60 57 59 1.92 3.2% Sample 2 251 287 247 277 265 16.93 6.3% Sample 3 939 986 993 986 976 21.55 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 58 60 57 59 1.92 3.2% Sample 1 62 58 60 57 59 1.92 3.2% Sample 2 251 287 247 277 265 16.93 6.3% Sample 2 251 287 247 277 265 16.93 6.3% Sample 3 939 986 993 986 976 21.55 2.2% Sample 3 939 986 993 986 976 21.55 2.2%
- Synonyms
- C-C motif chemokine 17; CC chemokine TARC; Small-inducible cytokine A17; Thymus and activation-regulated chemokine; CCL17; SCYA17, TARC
- Gene Name
- CCL17
- Gene ID
- 6361
- UniProt
- Q92583
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A24-S94
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7%
- Sensitivity
- <1 pg/mL
- Function
- Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10507 MW
- Specificity
- Natural and recombinant human CCL17
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 17
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Protein Name
- C-C motif chemokine 17
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
