- Catalog number
- EK0688
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK) . This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 98 95 92 92 4.43 4.8% Sample 2 240 249 256 245 247 5.85 2.3% Sample 3 846 852 955 890 885 43.39 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 98 95 92 92 4.43 4.8% Sample 1 86 98 95 92 92 4.43 4.8% Sample 2 240 249 256 245 247 5.85 2.3% Sample 2 240 249 256 245 247 5.85 2.3% Sample 3 846 852 955 890 885 43.39 4.9% Sample 3 846 852 955 890 885 43.39 4.9%
- Synonyms
- C-C motif chemokine 27; CC chemokine ILC; Cutaneous T-cell-attracting chemokine; CTACK; ESkine; IL-11 R-alpha-locus chemokine; Skinkine; Small-inducible cytokine A27; CCL27; ILC, SCYA27
- Gene Name
- CCL27
- Gene ID
- 10850
- UniProt
- Q9Y4X3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: F25-G112
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Testis, thymus, placenta, ovary and skin.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 12618 MW
- Specificity
- Natural and recombinant human CCL27
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 27
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Protein Name
- C-C motif chemokine 27
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
