- Catalog number
- EK0691
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 84 92 78 83 5.53 6.6% Sample 2 209 201 187 203 200 8.06 4% Sample 3 835 758 831 907 832 52.69 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 84 92 78 83 5.53 6.6% Sample 1 79 84 92 78 83 5.53 6.6% Sample 2 209 201 187 203 200 8.06 4% Sample 2 209 201 187 203 200 8.06 4% Sample 3 835 758 831 907 832 52.69 6.3% Sample 3 835 758 831 907 832 52.69 6.3%
- Synonyms
- C-C motif chemokine 28; Small-inducible cytokine A28; Ccl28; Scya28
- Gene Name
- CCL28
- Gene ID
- 56838
- UniProt
- Q9JIL2
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: I23-R130
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Mainly expressed in testis, epithelial cells of normal colon, kidney, Peyer patches, lymph nodes. Also found in lower levels in brain, spleen and lung.
- Applications
- ELISA
- Field of Research
- Chemokines, Cytokines, Immunology, Innate Immunity, Metabolism, Obesity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 209 835 80 199 814 Standard deviation 3.39 11.07 61.79 4.88 12.93 66.74 CV (%) 4.3% 5.3% 7.4% 6.1% 6.5% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 209 835 80 199 814 Standard deviation 3.39 11.07 61.79 4.88 12.93 66.74 CV (%) 4.3% 5.3% 7.4% 6.1% 6.5% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 209 835 80 199 814 Mean (pg/ml) 79 209 835 80 199 814 Standard deviation 3.39 11.07 61.79 4.88 12.93 66.74 Standard deviation 3.39 11.07 61.79 4.88 12.93 66.74 CV (%) 4.3% 5.3% 7.4% 6.1% 6.5% 8.2% CV (%) 4.3% 5.3% 7.4% 6.1% 6.5% 8.2%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic for resting CD4, CD8 T-cells and eosinophils (By similarity) . Binds to CCR10 and induces calcium mobilization in a dose-dependent manner. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14570 MW
- Specificity
- Natural and recombinant mouse CCL28
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 28
- Subcellular Location
- Secreted .
- Protein Name
- C-C motif chemokine 28
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.034 0.123 0.207 0.337 0.592 1.019 1.622 2.386 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.034 0.123 0.207 0.337 0.592 1.019 1.622 2.386 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.034 0.123 0.207 0.337 0.592 1.019 1.622 2.386 O.D. 0.034 0.123 0.207 0.337 0.592 1.019 1.622 2.386
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
